All the statistical programs gave similar EC(50) values, but GraphPad Prism® five-parameter analysis pointed out a best performance, also showing a minor variance in relation to the actual EC(50).Ĭopyright © 2012 Elsevier Ltd. ) assay to define the EC(50) parameters.For this project, quercetin, catechin, ascorbic acid, caffeic acid, chlorogenic acid and acetylcysteine were screened as antioxidant standards with DPPH( This method is accurate, easy to perform, and economical, providing a screening of the general activity of the antioxidants and is based in a stable and synthetic radical, DPPH 135. In this study, the EC(50) estimation was performed using a comparative approach based on various regression models implemented in six specialized computer programs: GraphPad Prism® version 5.01, BLeSq, OriginPro® 8.5.1, SigmaPlot® 12, BioDataFit® 1.02, and IBM SPSS Statistics® Desktop 19.0. One of the most popular colorimetric assays to estimate the radical scavenging capacity of plants and extracts is the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. ) radical scavenging activity is generally quantified in terms of inhibition percentage of the pre-formed free radical by antioxidants, and the EC(50) (concentration required to obtain a 50% antioxidant effect) is a typically employed parameter to express the antioxidant capacity and to compare the activity of different compounds.) assay is a reliable method to determine the antioxidant capacity of biological substrates.
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